Research

 

 

 

 

 

 

 

 

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The activities leading to these results have received funding from the European Union Seventh Framework Program (FP7/2007-2013) under grant agreement nr.  289159.

WP 2 Propagation, distribution and establishment of pre-selected plant material

Lead managed by Dr. Kai-Uwe Schwarz, Germany

 

WP2 is responsible for the in vitro propagation and distribution of pre-selected miscanthus plant material from existing miscanthus pools in the United Kingdom, Netherlands and Germany. The miscanthus collections in Aberystwyth (OPTIMISC partner IBERS, UK), Wageningen (WUR, the Netherlands) and Braunschweig (Schwarz, Germany) formed the basis for the selection of the genotypes for OPTIMISC field trials and laboratory experiments. Genotypes of different geographic origin from M. sinensis, M. sacchariflorus and M. floridulus and hybrids between these species have been selected and propagated.
During the second year  WP2 focused its efforts on the supply genotypes derived from in vitro culture for abiotic stress screening experiments and propagation of miscanthus for large-scale field experiments in Germany and Ukraine.
Until the end of 2013 over 150 miscanthus genotypes and accessions have been selected and propagated for the OPTIMISC experiments on various scales. All the partners involved in field and laborartory tests could be successfully supplied with sufficient plant material. About 100 genotypes have been successfully transferred to in vitro culture and distributed to project partners.
48,000 plants were propagated in 2013 for the large-scale trials established on 0.6 ha in Germany and on 1.8 ha in Ukraine.
The propagation of the plant material for the OPTIMISC experiments is based on in vitro culture techniques, rhizomes and seeds.

 

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Miscanthus in vitro propagation: (a) plants grown from callus; (b) plants grown from rhizome/nodal buds
(Photo: K.- U. Schwarz).

 

The expected final outcomes of the WP on propagation (WP2) will be the improvement of tissue culture techniques related to rooting of plants and the use of shoot tips in addition to nodal buds as a method of plant multiplication by direct in vitrotillering. Propagation methods will be compared with regard to their practicability, efficiency and commercialization.

 

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